Annexin V binding assay as a tool to measure apoptosis in differentiated neuronal cells. We describe a rapid and reliable method to quantitate the extent of apoptosis in neuronal cell cultures.

av GL Norddahl · 2011 · Citerat av 190 — cells were harvested and stained with Annexin V and/or propidium iodide (PI) to measure apoptosis. These experiments revealed that HSCs

T RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay 1,000 assays JA1012 Contains sufficient reagents to perform 1,000 assays in a 96-well plate format or 4,000 assays in a 384-well plate format. Includes: Successful detection of early apoptosis with Annexin V-FITC is dependent upon several factors including cell type and level of membrane PS, amount of PS that is exposed during apoptosis, method (or agent) inducing apoptosis, time of apoptotic induction, as well as degree of mechanical manipulation. Annexin Vはアポトーシスにより膜表面に露出したPSに結合します。. これによりアポトーシス細胞を検出することできます。.

Maastricht Universitet [Annexin V levels in survivors of early myocardial infarction]. Annexin V has a strong, Ca 2 +-dependent affinity for PS and therefore can be used as a probe for detecting apoptosis. Save time with a validated Annexin V assay kit/reagent. For robust results, we recommend our range of one-step Annexin V staining/detection kits and reagents for use in flow cytometry and cell imaging. Choose from Annexin V conjugated to FITC, Cy5, Cy3, PE, iFluor 488, biotin, or several other popular fluorescent dyes.

av C Trinks · 2010 · Citerat av 15 — ErbB-independent apoptosis in human leukemia (HL-60 and U-937) cells. more induced apoptosis as determined by the Annexin V method and cleavage of

17 rows Annexin V, a 36-kDa calcium-binding protein, binds to PS; therefore, fluorescently labeled Annexin V can be used to detect PS that is exposed on the outside of apoptotic cells. Annexin V can also stain necrotic cells because these cells have ruptured membranes that permit Annexin V to access the entire plasma membrane. Annexin V apoptosis assay.

Development of the Annexin V Apoptosis Assay: • 1990 Andree at al. found that a protein, Vascular Anticoagulant α, bound to phospholipid bilayers in a calcium

This study evaluated the efficacy of (111)In-labeled Annexin V for monitoring tumor responses during prodrug activation gene ther 2017-05-25 One vial of lyophilized solid sufficient for 100-200 live-cell apoptosis tests in 96-well format. The Incucyte® Annexin V Red Dye is a highly-selective phosphatidylserine (PS) cyanine fluorescent dye that is specially formulated and ideally suited for real-time measurements of apoptosis in living cultures. The … The RealTime-Glo™ Annexin V Apoptosis and Necrosis Assay measures the real-time exposure of phosphatidylserine (PS) on the outer leaflet of cell membranes during the apoptotic process. Annexin V luciferase fusion proteins supplied in the assay reagent bind to PS during early apoptosis and are detected with a simple luminescence signal. 4th figure: add apoptosis inducing agent staurosporine and stain with both Annexin V and PI 5th figure: no inducing reagent but stain both Annexin V and PI I don't know how to interpret my result.

PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during apoptosis, PS translocates to the external leaflet. Description: ApoScreen ® Annexin V Apoptosis Kit-PE (SB Cat. No. 10010-09) employs a R-phycoerythrin-conjugated Annexin V (Annexin V-PE) in concert with 7-Aminoactinomycin D (7-AAD) to evaluate subpopulations of cells undergoing apoptosis. Annexin V-FITC Apoptosis Detection Kit (Catalog #: K101-25, -100, -400; Store at 4oC; Stable for one year) I. Introduction: Annexin V Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. ApopNexin Annexin V FITC Apoptosis Kit The ApopNexin FITC Apoptosis Detection Kit for Flow Cytometry is designed to detect a specific biochemical change in FITC Annexin V staining precedes the loss of membrane integrity which accompanies the latest stages of cell death resulting from either apoptotic or necrotic Development of the Annexin V Apoptosis Assay: • 1990 Andree at al. found that a protein, Vascular Anticoagulant α, bound to phospholipid bilayers in a calcium 3 Mar 2019 The Annexin V Apoptosis Detection Kit utilizes FITC-conjugated Annexin V protein for detecting cells undergoing apoptosis. Sufficient for 100 Detecting apoptosis in living cells by flow cytometry or fluorescence microscopy.
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Application 1 Fluorescent imaging of apoptosis induced cells Application 2 Annexin V is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. In healthy cells PS is normally only found on the intracellular leaflet of the plasma membrane, but during apoptosis, PS translocates to the external leaflet.

Annexin V apoptosis assay.
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99m Tc-ANNexin-V-128 Scintigraphy for the Identification of Complicated Carotid an ischemic stroke Cellular apoptosis is a key feature of ruptured plaques.

The kit is used for the combined detection of early-stage cell apoptosis (using Annexin V) and late-stage cell apoptosis or necrosis (using both Annexin V and 7-AAD). Annexin V-FITC Apoptosis Detection Kit II This annexin FITC Apoptosis detection kit is a highly sensitive and specific fluorometric assay kit for measuring Annexin V in apoptotic cells. - Find MSDS or SDS, a COA, data sheets and more information. 4th figure: add apoptosis inducing agent staurosporine and stain with both Annexin V and PI 5th figure: no inducing reagent but stain both Annexin V and PI I don't know how to interpret my result.